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Riprogrammazione primario del liquido amniotico e le celle a membrana di pluripotenza in condizioni prive di Xeno
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09:34 min
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November 27, 2017
DOI:
1Mitchell Cancer Institute,University of South Alabama, 2College of Medicine,University of South Alabama, 3Institute for Regenerative Medicine,University of Zurich, 4Department of Dermatology,University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM),University of Zurich – Irchel Campus
Summary
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Questo protocollo descrive la riprogrammazione di primaria amniotico liquido e membrana di cellule staminali mesenchimali in cellule staminali pluripotenti indotte utilizzando un approccio episomal non-integrazione in condizioni completamente chimicamente definite. Procedure di estrazione, cultura, riprogrammazione e caratterizzazione delle cellule staminali pluripotenti indotte risultanti dai rigorosi metodi sono dettagliate.
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Keyword Extraction:Amniotic FluidMembrane CellsPluripotencyXeno-free ConditionsInduced Pluripotent Stem CellsEpisomal ReprogrammingFetal Stem CellsDisease ModelingBasic ResearchLongitudinal StudiesChemically Defined ConditionsPediatric Cell-based TherapiesPlacentasAmnionRPMI-1640 MediumAFMC MediumAmniotic Fluid Stem CellsMembrane Stem CellsCell Detachment EnzymePBS
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Slamecka, J., Laurini, J., Shirley, T., Hoerstrup, S. P., Weber, B., Owen, L., McClellan, S. Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions. J. Vis. Exp. (129), e56003, doi:10.3791/56003 (2017).
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