Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Oriana Romano; Ingrid Cifola; Valentina Poletti; Marco Severgnini; Clelia Peano; Gianluca De Bellis; Fulvio Mavilio; Annarita Miccio

doi:10.3791/55919

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Génétique

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Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

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Génétique

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Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

8,307 Views

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10:10 min

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May 28, 2017

DOI:

10.3791/55919-v

DOI:

10.3791/55919-v

•

10:10 min

May 28, 2017

•

2 Views

Oriana Romano^1,2, Ingrid Cifola³, Valentina Poletti⁴, Marco Severgnini³, Clelia Peano³, Gianluca De Bellis³, Fulvio Mavilio^1,4, Annarita Miccio^1,2,4,5

¹Center for Genome Research, Department of Life Sciences,University of Modena and Reggio Emilia, ²Laboratory of Chromatin and Gene Regulation During Development,Imagine Institute, ³Institute for Biomedical Technologies,CNR, ⁴Généthon, ⁵Sorbonne Paris Cité – Université Paris Descartes

Summary

Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.

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Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

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