Dergi
/
Genetik
/
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
JoVE Journal
Genetik
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Genetik
Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions

8,307 Views

•

10:10 min

•

May 28, 2017

DOI:

10.3791/55919-v

DOI:
10.3791/55919-v

•

10:10 min
May 28, 2017

•

2 Views
, , , , , , ,
1Center for Genome Research, Department of Life Sciences,University of Modena and Reggio Emilia, 2Laboratory of Chromatin and Gene Regulation During Development,Imagine Institute, 3Institute for Biomedical Technologies,CNR, 4Généthon, 5Sorbonne Paris Cité – Université Paris Descartes

Özet

Automatically generated

Here, we describe a protocol for genome-wide mapping of the integration sites of Moloney murine leukemia virus-based retroviral vectors in human cells.

Explore More Videos

Retroviral ScanningMLV Integration SitesCell-specific Regulatory RegionsGene TherapyGene RegulationMLV-derived Retroviral VectorEGFP Reporter GeneFlow Cytometric AnalysisGenomic DNA PreparationRestriction Enzyme DigestionLinker LigationFirst-round PCRSecond-round PCR

Read Article

Cite this Article

Romano, O., Cifola, I., Poletti, V., Severgnini, M., Peano, C., De Bellis, G., Mavilio, F., Miccio, A. Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions. J. Vis. Exp. (123), e55919, doi:10.3791/55919 (2017).

Download .ris file

Copy

Share Video

.

Copy