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Journal
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Biochemistry
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一种简单的分级提取方法,用于从单个样品中综合分析代谢物,脂质和蛋白质
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
A Simple Fractionated Extraction Method for the Comprehensive Analysis of Metabolites, Lipids, and Proteins from a Single Sample
Please note that all translations are automatically generated.
Click here for the English version.
一种简单的分级提取方法,用于从单个样品中综合分析代谢物,脂质和蛋白质
DOI:
10.3791/55802-v
•
11:17 min
•
June 01, 2017
•
Mohamed Salem
2
,
Michal Bernach
,
Krzysztof Bajdzienko
,
Patrick Giavalisco
1
Max Planck Institute of Molecular Plant Physiology, Golm, Germany
,
2
Department of Pharmacognosy, Faculty of Pharmacy
,
Cairo University
Chapters
00:05
Title
01:05
Grinding and Tissue Disruption
02:30
Aliquoting Tissue
03:18
Extraction and Fractionation by Phase Separation
04:56
Aliquoting Polar and Hydrophobic Fractions
06:24
Concentrations and Storage of Fraction and Analysis of Lipids Using UPLC-MS
07:48
Analysis of Polar and Semi-polar Metabolites Using UPLC-MS
08:51
Results: Representative Chromatograms of Lipid, Semi-polar Metabolites and Proteins from
Arabidopsis thaliana
Leaf Extracts
10:40
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了使用一个样品从生物组织中综合提取脂质,代谢物和蛋白质的方案。
Tags
Fractionated Extraction
Metabolites
Lipids
Proteins
Single Sample
MTBE Extraction
Multiomics Analysis
Arabidopsis
Sample Preparation
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