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Neuroscience
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时间依赖性增加对微电极阵列刺激神经元细胞培养物的网络响应
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal
Neuroscience
Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays
Please note that all translations are automatically generated.
Click here for the English version.
时间依赖性增加对微电极阵列刺激神经元细胞培养物的网络响应
DOI:
10.3791/55726-v
•
10:45 min
•
May 29, 2017
•
Monica L. Gertz
,
Zachary Baker
,
Sharon Jose
,
Nathalia Peixoto
1
Krasnow Institute for Advanced Study
,
George Mason University
,
2
Neural Engineering, Bioengineering
,
George Mason University
,
3
Neural Engineering, Computer Science
,
George Mason University
,
4
Electrical and Computer Engineering
,
George Mason University
Chapters
00:05
Title
01:04
Embryo Removal and Brain Extraction
03:19
Frontal Cortext Removal
04:46
Cell Dissociation
06:48
Plating Cells and Maintaining the Cultures
08:01
Visual Inspections and Recording
09:24
Results: Trained Networks Have Significantly Altered Spike Frequencies
10:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
在多电极阵列上培养的小鼠神经元细胞在电刺激后显示出响应的增加。该协议演示了如何培养神经元,如何记录活动,以及如何建立协议来训练网络来应对刺激模式。
Tags
Microelectrode Arrays
Neuronal Cell Cultures
Network Dynamics
Learning And Memory
Drug Toxicity
Personalized Medicine
Dissection
Dissociation
Laminin
L-15 Medium
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