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March 16, 2015
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We present a method for the electroretinographic (ERG) analysis of zebrafish larvae utilizing micromanipulation and electroretinography techniques. This is a simple and straightforward method for assaying visual function of zebrafish larvae in vivo.
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ElectroretinogramERGZebrafish LarvaeRetinal FunctionCone Visual FunctionIn VivoNoninvasive ElectrophysiologyGene SuppressionMorpholino OligonucleotidesPharmacological ManipulationStereomicroscopyElectrophysiology EquipmentLow Light CameraERG Stimulator/recorderZinc Finger Nucleases (ZFNs)Transcription Activator-Like Effector Nucleases (TALENs)Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9Pharmacological Agents
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Chrispell, J. D., Rebrik, T. I., Weiss, E. R. Electroretinogram Analysis of the Visual Response in Zebrafish Larvae. J. Vis. Exp. (97), e52662, doi:10.3791/52662 (2015).
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