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Journal
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Developmental Biology
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诱导生活青蛙和斑马鱼胚胎缺氧
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Induction of Hypoxia in Living Frog and Zebrafish Embryos
Please note that all translations are automatically generated.
Click here for the English version.
诱导生活青蛙和斑马鱼胚胎缺氧
DOI:
10.3791/55710-v
•
08:01 min
•
June 26, 2017
•
Helena Khaliullina-Skultety
,
Ngiam Zi Chao
,
William A. Harris
1
Department of Physiology, Development and Neuroscience
,
University of Cambridge
Chapters
00:05
Title
00:46
Induction of Hypoxia
In Vivo
02:40
Induction of Hypoxia in Embryos and Collection of Tissue to Monitor HIF-1α Levels
04:19
Incubation of Embryos in Hypoxia for EdU Incorporation
05:13
Results: The Effects of Hypoxia on HIF-1α and on Cell Proliferation
06:42
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们引进了一种新颖的低氧室系统,用于水生生物,如青蛙和斑马鱼胚胎。我们的系统简单,健壮,经济实惠,可以
在体内
诱导和维持缺氧
,
长达48小时。我们提供2种可重复的方法来监测缺氧的有效性。
Tags
Hypoxia
Hyperoxia
Aquatic Organisms
Developmental Biology
Cell Biology
Embryonic Growth Restriction
Fetal Hypoxia
High-altitude Adaptation
Obesity
Solid Tumor Development
Xenopus
Zebrafish
24-well Plate
Gas Tank
Oxygen-nitrogen Mixture
Gas Regulator
Ceramic Disk Diffuser
Oxygen Concentration
Oximeter
Oxygen Probe
Temperature Incubator
Embryo Transfer
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