Journal
/
/
诱导生活青蛙和斑马鱼胚胎缺氧
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Induction of Hypoxia in Living Frog and Zebrafish Embryos
DOI:

08:01 min

June 26, 2017

, ,

Chapters

  • 00:05Title
  • 00:46Induction of Hypoxia In Vivo
  • 02:40Induction of Hypoxia in Embryos and Collection of Tissue to Monitor HIF-1α Levels
  • 04:19Incubation of Embryos in Hypoxia for EdU Incorporation
  • 05:13Results: The Effects of Hypoxia on HIF-1α and on Cell Proliferation
  • 06:42Conclusion

Summary

Automatic Translation

我们引进了一种新颖的低氧室系统,用于水生生物,如青蛙和斑马鱼胚胎。我们的系统简单,健壮,经济实惠,可以在体内诱导和维持缺氧长达48小时。我们提供2种可重复的方法来监测缺氧的有效性。

Related Videos

Read Article