Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Induction of Hypoxia in Living Frog and Zebrafish Embryos
9,527 Views
•
08:01 min
•
June 26, 2017
DOI:
Summary
Automatically generated
We introduce a novel hypoxic chamber system for use with aquatic organisms such as frog and zebrafish embryos. Our system is simple, robust, cost-effective and allows the induction and sustainment of hypoxia in vivo and for up to 48 h. We present 2 reproducible methods to monitor the effectiveness of hypoxia.
Explore More Videos
HypoxiaHyperoxiaAquatic OrganismsBiología del desarrolloCell BiologyEmbryonic Growth RestrictionFetal HypoxiaHigh-altitude AdaptationObesitySolid Tumor DevelopmentXenopusZebrafish24-well PlateGas TankOxygen-nitrogen MixtureGas RegulatorCeramic Disk DiffuserOxygen ConcentrationOximeterOxygen ProbeTemperature IncubatorEmbryo Transfer
Read Article
Cite this Article
Khaliullina-Skultety, H., Zi Chao, N., Harris, W. A. Induction of Hypoxia in Living Frog and Zebrafish Embryos. J. Vis. Exp. (124), e55710, doi:10.3791/55710 (2017).
Copy