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Isolatie van intermediaire filamentproteïnen uit meerdere muisweefsels om te bestuderen veroudering-geassocieerde post-translationele modificaties
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Biochemistry
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JoVE Journal Biochemistry
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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Isolatie van intermediaire filamentproteïnen uit meerdere muisweefsels om te bestuderen veroudering-geassocieerde post-translationele modificaties

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8,352 Views

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09:29 min

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May 18, 2017

DOI:

10.3791/55655-v

DOI:
10.3791/55655-v

•

09:29 min
May 18, 2017

•

1 Views
, , , ,
1Department of Cell Biology and Physiology, School of Medicine,University of North Carolina at Chapel Hill

Summary

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In deze methode presenteren wij biochemische procedures voor snelle en efficiënte isolatie van intermediaire filament (IF) eiwitten uit meerdere muisweefsels. Geïsoleerde IF's kunnen worden gebruikt om veranderingen te onderzoeken in post-translatie modificaties door massaspectrometrie en andere biochemische analyses.

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Cite this Article

Battaglia, R. A., Kabiraj, P., Willcockson, H. H., Lian, M., Snider, N. T. Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications. J. Vis. Exp. (123), e55655, doi:10.3791/55655 (2017).

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