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Biochemistry

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JoVE Journal Biochemistry
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
 

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Article DOI: 10.3791/55655-v • 09:29 min • May 18th, 2017
May 18th, 2017 •
1, 1, 1, 1, 1
1Department of Cell Biology and Physiology, School of Medicine, University of North Carolina at Chapel Hill

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In this method, we present biochemical procedures for rapid and efficient isolation of intermediate filament (IF) proteins from multiple mouse tissues. Isolated IFs can be used to study changes in post-translational modifications by mass spectrometry and other biochemical assays.

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Isolation Intermediate Filament Proteins Mouse Tissues Aging-associated Post-translational Modifications Biochemical Procedure Enriched Fractions Study Function Regulation Stress Protective Cytoskeletal Proteins Mammalian Aging Automated Tissue Lysis Step Rapid And Efficient Manner Triton X-100 Buffer Protease Inhibitors Glass Tube Homogenizer Ice Cold Liquid Nitrogen Storage Polytetrafluoroethylene Pastille Sample Homogenization Formation Of Bubbles Microcentrifuge Tube Centrifugation Triton X Soluble Supernatant Fraction Immuno Precipitation
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