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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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May 18, 2017
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In this method, we present biochemical procedures for rapid and efficient isolation of intermediate filament (IF) proteins from multiple mouse tissues. Isolated IFs can be used to study changes in post-translational modifications by mass spectrometry and other biochemical assays.
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Battaglia, R. A., Kabiraj, P., Willcockson, H. H., Lian, M., Snider, N. T. Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications. J. Vis. Exp. (123), e55655, doi:10.3791/55655 (2017).
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