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Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
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JoVE Journal Biochimica
Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

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09:29 min

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May 18, 2017

DOI:

10.3791/55655-v

DOI:
10.3791/55655-v

•

09:29 min
May 18, 2017

•

2 Views
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1Department of Cell Biology and Physiology, School of Medicine,University of North Carolina at Chapel Hill

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Intermediate Filament ProteinsPost-translational ModificationsTriton X-100 BufferHigh Salt BufferPBS EDTA BufferSDS Sample BufferCentrifugationHomogenization

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Battaglia, R. A., Kabiraj, P., Willcockson, H. H., Lian, M., Snider, N. T. Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications. J. Vis. Exp. (123), e55655, doi:10.3791/55655 (2017).

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