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Measuring Protein Binding to F-actin by Co-sedimentation
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May 18, 2017
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This protocol describes a method to test the ability of a protein to co-sediment with filamentous actin (F-actin) and, if binding is observed, to measure the affinity of the interaction.
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Heier, J. A., Dickinson, D. J., Kwiatkowski, A. V. Measuring Protein Binding to F-actin by Co-sedimentation. J. Vis. Exp. (123), e55613, doi:10.3791/55613 (2017).
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