/
/
Small RNA Transfección en células primarias Th17 humano por Next Generation electroporación
A subscription to JoVE is required to view this content. Sign in or start your free trial.
7,717 Views
•
10:15 min
•
April 13, 2017
DOI:
Summary
Automatically generated
Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.
Explore More Videos
Small RNA TransfectionPrimary Human Th17 CellsNext Generation ElectroporationT Cell BiologyGene RegulationT Cell DifferentiationImmune FunctionHuman Th17 CellsLymphocytesCord-blood Mononuclear CellsCD4 Positive T Cell IsolationNegative SelectionMagnetic BeadsCell CultureCell IsolationCell WashingCell IncubationCell Resuspension
Read Article
Cite this Article
Montoya, M. M., Ansel, K. M. Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation. J. Vis. Exp. (122), e55546, doi:10.3791/55546 (2017).
Copy