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Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

DOI:
10.3791/55546-v

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10:15 min

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April 13, 2017

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,
1Department of Microbiology & Immunology, Sandler Asthma Basic Research Center,University of California, San Francisco

Kapitel

  • 00:05Titel
  • 00:48T Cell Culture Preparation and CD4+ T Cell Isolation
  • 03:38Th17 Polarization
  • 04:41Electroporation of In Vitro Polarized Human Th17 Cells
  • 08:03Results: Robust In Vitro Differentiated Human Th17 Cell Cultures
  • 09:48Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.

Tags

Small RNA TransfectionPrimary Human Th17 CellsNext Generation ElectroporationT Cell BiologyGene RegulationT Cell DifferentiationImmune FunctionHuman Th17 CellsLymphocytesCord-blood Mononuclear CellsCD4 Positive T Cell IsolationNegative SelectionMagnetic BeadsCell CultureCell IsolationCell WashingCell IncubationCell Resuspension

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