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Piccolo RNA trasfezione in cellule primarie Th17 Human Next Generation elettroporazione
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April 13, 2017
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Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.
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Small RNA TransfectionPrimary Human Th17 CellsNext Generation ElectroporationT Cell BiologyGene RegulationT Cell DifferentiationImmune FunctionHuman Th17 CellsLymphocytesCord-blood Mononuclear CellsCD4 Positive T Cell IsolationNegative SelectionMagnetic BeadsCell CultureCell IsolationCell WashingCell IncubationCell Resuspension
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Montoya, M. M., Ansel, K. M. Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation. J. Vis. Exp. (122), e55546, doi:10.3791/55546 (2017).
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