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Immunologie et infection

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Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

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Immunologie et infection

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Journal JoVE Immunologie et infection

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

7,708 Views

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10:15 min

•

April 13, 2017

DOI:

10.3791/55546-v

DOI:

10.3791/55546-v

•

10:15 min

April 13, 2017

•

2 Views

Misty M. Montoya, K. Mark Ansel

¹Department of Microbiology & Immunology, Sandler Asthma Basic Research Center,University of California, San Francisco

Tags

Small RNA Transfection Primary Human Th17 Cells Next Generation Electroporation T Cell Biology Gene Regulation T Cell Differentiation Immune Function Human Th17 Cells Lymphocytes Cord-blood Mononuclear Cells CD4 Positive T Cell Isolation Negative Selection Magnetic Beads Cell Culture Cell Isolation Cell Washing Cell Incubation Cell Resuspension

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