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Serebellar Granül Nöron Kültüründe Floresan Diasetat-Propidyum İyodür Çift Boyama Kullanarak Nöronal Canlılığın Değerlendirilmesi
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08:53 min
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May 10, 2017
DOI:
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Summary
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Bu protokol, nörobilim ve nörofarmakoloji araştırmalarında in vitro bir model olarak kullanılan birincil nöronal kültür olan kültürlenmiş serebellar granül nöronlarında Fluorescein diasetat (FDA) ve Propidyum İyodür (PI) çift boyasını kullanarak nöronal canlılığı nasıl doğru bir şekilde ölçtüğümü açıklamaktadır.
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Cite this Article
Jiajia, L., Shinghung, M., Jiacheng, Z., Jialing, W., Dilin, X., Shengquan, H., Zaijun, Z., Qinwen, W., Yifan, H., Wei, C. Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture. J. Vis. Exp. (123), e55442, doi:10.3791/55442 (2017).
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