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Évaluation de la viabilité neuronale à l'aide de diacétate de fluorescéine-iodure de propidium Double coloration dans la culture du neurone de granulé céréalier
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08:53 min
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May 10, 2017
DOI:
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
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Ce protocole décrit comment mesurer avec précision la viabilité neuronale à l'aide de la diurétate de fluorescéine (FDA) et de la double coloration à l'iodure de propidium (PI) dans des neurones de granules cérébelleux cultivés, une culture neuronale primaire utilisée comme modèle in vitro dans les recherches en neuroscience et neuropharmacologie.
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Cite this Article
Jiajia, L., Shinghung, M., Jiacheng, Z., Jialing, W., Dilin, X., Shengquan, H., Zaijun, Z., Qinwen, W., Yifan, H., Wei, C. Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture. J. Vis. Exp. (123), e55442, doi:10.3791/55442 (2017).
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