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Beoordeling van neuronale levensvatbaarheid met behulp van Fluorescein Diacetate-Propidium Iodide Dubbele Verf In Cerebellaire Granule Neuron Cultuur
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08:53 min
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May 10, 2017
DOI:
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Summary
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Dit protocol beschrijft hoe om neuronale levensvatbaarheid nauwkeurig te meten met behulp van Fluorescein Diacetate (FDA) en Propidium Iodide (PI) dubbele kleuring in gekweekte cerebellar granule neuronen, een primaire neuronale cultuur die gebruikt wordt als een in vitro model in neurowetenschappen en neurofarmacologie onderzoek.
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Cite this Article
Jiajia, L., Shinghung, M., Jiacheng, Z., Jialing, W., Dilin, X., Shengquan, H., Zaijun, Z., Qinwen, W., Yifan, H., Wei, C. Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture. J. Vis. Exp. (123), e55442, doi:10.3791/55442 (2017).
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