/
/
Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture
11,440 Views
•
08:53 min
•
May 10, 2017
DOI:
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine,Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine,The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy,Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
Summary
Automatically generated
This protocol describes how to accurately measure neuronal viability using Fluorescein diacetate (FDA) and Propidium Iodide (PI) double staining in cultured cerebellar granule neurons, a primary neuronal culture used as an in vitro model in neuroscience and neuropharmacology research.
Explore More Videos
Neuronal ViabilityFluorescein Diacetate-propidium IodideCerebellar Granule Neuron CultureCell ViabilityNeurowissenschaftenNeuropharmacologyGlial CellsNeuronsMISTA Cell CultureRat PupsCerebellumDissectionCentrifugationTrypsinDNaseSoybean Trypsin InhibitorMagnesium SulfateCalcium ChlorideCell Density6-well PlateCell Culture
Read Article
Cite this Article
Jiajia, L., Shinghung, M., Jiacheng, Z., Jialing, W., Dilin, X., Shengquan, H., Zaijun, Z., Qinwen, W., Yifan, H., Wei, C. Assessment of Neuronal Viability Using Fluorescein Diacetate-Propidium Iodide Double Staining in Cerebellar Granule Neuron Culture. J. Vis. Exp. (123), e55442, doi:10.3791/55442 (2017).
Copy