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Detección y enriquecimiento de Rare células B específicas de antígeno para el análisis del fenotipo y la función
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09:25 min
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February 16, 2017
DOI:
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology,Colorado State University, 3Barbara Davis Center for Childhood Diabetes,University of Colorado School of Medicine, 4Department of Biomedical Research,National Jewish Health
Summary
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Un método simple y efectivo que emplea nanopartículas magnéticas para detectar y enriquecer células B reactivas al antígeno para el análisis funcional y fenotípica se describe.
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Smith, M. J., Packard, T. A., O'Neill, S. K., Hinman, R. M., Rihanek, M., Gottlieb, P. A., Cambier, J. C. Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function. J. Vis. Exp. (120), e55382, doi:10.3791/55382 (2017).
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