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Detecção e enriquecimento da Rare células B antígeno-específica para a análise do fenótipo e função
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09:25 min
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February 16, 2017
DOI:
1Department of Immunology and Microbiology,University of Colorado School of Medicine, 2Department of Microbiology, Immunology, and Pathology,Colorado State University, 3Barbara Davis Center for Childhood Diabetes,University of Colorado School of Medicine, 4Department of Biomedical Research,National Jewish Health
Summary
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Um método simples, mas eficaz, que emprega nanopartículas magnéticas para detectar e enriquecer as células B antígeno-reativa para análise funcional e fenotípica é descrito.
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Smith, M. J., Packard, T. A., O'Neill, S. K., Hinman, R. M., Rihanek, M., Gottlieb, P. A., Cambier, J. C. Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function. J. Vis. Exp. (120), e55382, doi:10.3791/55382 (2017).
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