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Developmental Biology
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界定和由人多能干细胞的肝细胞样细胞的可扩展代
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
界定和由人多能干细胞的肝细胞样细胞的可扩展代
DOI:
10.3791/55355-v
•
08:36 min
•
March 02, 2017
•
Yu Wang
,
Sharmin Alhaque
,
Kate Cameron
,
Jose Meseguer-Ripolles
,
Baltasar Lucendo-Villarin
,
Hassan Rashidi
,
David C. Hay
1
MRC Centre for Regenerative Medicine
,
University of Edinburgh
Chapters
00:05
Title
00:36
Passaging Human Pluripotent Stem Cells (hPSCs)
04:14
Differentiating hPSCs to Hepatocyte-like Cells on Recombinant Laminins
06:46
Results: Superior Hepatocyte Differentiation Was Achieved on Laminin Substrates
08:05
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的方法描述了一个可扩展性和良好生产规范(GMP)-ready分化系统生成的多能干细胞的人类肝细胞样细胞。它作为一个具有成本效益的和标准化的系统以产生用于基础和应用的人肝研究人肝细胞样细胞。
Tags
Human Pluripotent Stem Cells
Hepatocyte-like Cells
Defined Conditions
Recombinant Laminin Matrices
Differentiation Protocol
Cell Culture
Scalable Production
Biomedical Research
Cell-based Modeling
Cell Therapy
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