The method presented here describes a scalable and good manufacturing practice (GMP)-ready differentiation system to generate human hepatocyte-like cells from pluripotent stem cells. It serves as a cost-effective and standardized system to generate human hepatocyte-like cells for basic and applied human liver research.
Wang, Y., Alhaque, S., Cameron, K., Meseguer-Ripolles, J., Lucendo-Villarin, B., Rashidi, H., Hay, D. C. Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells. J. Vis. Exp. (121), e55355, doi:10.3791/55355 (2017).