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发育生物学
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Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
JoVE 杂志
发育生物学
This content is Free Access.
JoVE 杂志 发育生物学
Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

DOI:
10.3791/55355-v

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08:36 min

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March 02, 2017

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, , , , , ,
1MRC Centre for Regenerative Medicine,University of Edinburgh

Chapters

  • 00:05Title
  • 00:36Passaging Human Pluripotent Stem Cells (hPSCs)
  • 04:14Differentiating hPSCs to Hepatocyte-like Cells on Recombinant Laminins
  • 06:46Results: Superior Hepatocyte Differentiation Was Achieved on Laminin Substrates
  • 08:05Conclusion

Summary

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The method presented here describes a scalable and good manufacturing practice (GMP)-ready differentiation system to generate human hepatocyte-like cells from pluripotent stem cells. It serves as a cost-effective and standardized system to generate human hepatocyte-like cells for basic and applied human liver research.

Tags

Human Pluripotent Stem CellsHepatocyte-like CellsDefined ConditionsRecombinant Laminin MatricesDifferentiation ProtocolCell CultureScalable ProductionBiomedical ResearchCell-based ModelingCell Therapy

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