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Solea Senegalensis精子中活性氧的细胞内定位评价
JoVE Journal
Developmental Biology
Author Produced
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JoVE Journal Developmental Biology
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
DOI:

10:24 min

March 11, 2018

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Chapters

  • 00:00Title
  • 01:32Preparatory work before the experiment
  • 02:58Sample preparation before fluorochromes incubation
  • 04:40Fluorochromes incubation
  • 05:33Sample preparation for confocal microscopy
  • 05:51Confocal setting up prior to the experiment
  • 06:49Acquisition of images
  • 07:49Results
  • 08:24Creating a 3D image video
  • 09:53Conclusions

Summary

Automatic Translation

此协议描述了一个用于检测Solea senegalensis精子中的 H2O2本地化的详细方法, 使用敏感的 fluorochrome DCFH-DA 为 ROS、线粒体的活线粒体染色和细胞核的 DAPI可视化, 分别。该协议的设计是在2小时内进行, 无论是新鲜或解冻精子。

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