Differential Scanning Calorimetry &#8212; A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Ibrahim B. Durowoju; Kamaljit S. Bhandal; Jian Hu; Bruce Carpick; Marina Kirkitadze

doi:10.3791/55262

Journal

/

Biochemistry

/

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

37,712 Views

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08:13 min

•

March 04, 2017

DOI:

10.3791/55262-v

DOI:

10.3791/55262-v

•

08:13 min

March 04, 2017

•

47 Views

Ibrahim B. Durowoju¹, Kamaljit S. Bhandal¹, Jian Hu¹, Bruce Carpick¹, Marina Kirkitadze¹

¹Analytical Research & Development,Sanofi Pasteur Limited

Summary

Differential scanning calorimetry measures the thermal transition temperature(s) and total heat energy required to denature a protein. Results obtained are used to assess the thermal stability of protein antigens in vaccine formulations.

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Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

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