Differential scanning calorimetry measures the thermal transition temperature(s) and total heat energy required to denature a protein. Results obtained are used to assess the thermal stability of protein antigens in vaccine formulations.
Durowoju, I. B., Bhandal, K. S., Hu, J., Carpick, B., Kirkitadze, M. Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen. J. Vis. Exp. (121), e55262, doi:10.3791/55262 (2017).