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Propagação em pequena escala de iPSCs humano em condições sem soro para a caracterização imunocitoquímica de rotina
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February 18, 2017
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Summary
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Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.
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Corenblum, M. J., Madhavan, L. Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization. J. Vis. Exp. (120), e55260, doi:10.3791/55260 (2017).
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