需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
6,866 Views
•
09:56 min
•
February 18, 2017
DOI:
Summary
Automatically generated
Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.
Read Article
Cite this Article
Corenblum, M. J., Madhavan, L. Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization. J. Vis. Exp. (120), e55260, doi:10.3791/55260 (2017).
Copy