Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.
Corenblum, M. J., Madhavan, L. Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization. J. Vis. Exp. (120), e55260, doi:10.3791/55260 (2017).