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Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization

Small-scale Propagation of Human iPSCs in Serum-free Conditions for Routine Immunocytochemical Characterization

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09:56 min

February 18, 2017

DOI:

09:56 min
February 18, 2017

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Summary

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Regular characterization of induced pluripotent stem cells (iPSCs), to ascertain maintenance of their pluripotent state, is an important step before these cells are used for other applications. Here we describe a method for the small-scale propagation of human iPSCs specifically designed to enable their easy and routine characterization via immunocytochemistry.

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