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Bottlenose Dolphin (Tursiops truncatus) espermatozoides: recolha, criopreservação e heterólogo fertilização In Vitro
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August 21, 2017
DOI:
1Department of Animal Reproduction,Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 2Department of Animal Medicine and Surgery, School of Veterinary Medicine,Universidad Complutense de Madrid, 3Department of Physiology, Faculty of Veterinary Science,University of Murcia, Campus Mare Nostrum, 4Mundomar, Benidorm, 5Veterinary Services,L’Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013
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Aqui, apresentamos os protocolos que foram usados com sucesso para a coleta de espermatozoides de golfinho, criopreservação e desempenho de fertilização in vitro heterólogo usando oócitos bovinos.
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Cite this Article
Sánchez-Calabuig, M. J., García-Vázquez, F. A., Laguna-Barraza, R., Barros-García, C., García-Parraga, D., Rizos, D., Gutiérrez Adan, A., Pérez-Gutíerrez, J. F. Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization. J. Vis. Exp. (126), e55237, doi:10.3791/55237 (2017).
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