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Journal
/
Developmental Biology
/
宽吻海豚 (
海豚截形
) 精子: 收集、 冷冻保存和外源
体外
受精
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Bottlenose Dolphin (
Tursiops truncatus
) Spermatozoa: Collection, Cryopreservation, and Heterologous
In Vitro
Fertilization
Please note that all translations are automatically generated.
Click here for the English version.
宽吻海豚 (
海豚截形
) 精子: 收集、 冷冻保存和外源
体外
受精
DOI:
10.3791/55237-v
•
11:42 min
•
August 21, 2017
•
María Jesús Sánchez-Calabuig
2
,
Francisco Alberto García-Vázquez
,
Ricardo Laguna-Barraza
,
Carlos Barros-García
,
Daniel García-Parraga
,
Dimitrios Rizos
,
Alfonso Gutiérrez Adan
,
José Félix Pérez-Gutíerrez
1
Department of Animal Reproduction
,
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
,
2
Department of Animal Medicine and Surgery, School of Veterinary Medicine
,
Universidad Complutense de Madrid
,
3
Department of Physiology, Faculty of Veterinary Science
,
University of Murcia, Campus Mare Nostrum
,
4
Mundomar, Benidorm
,
5
Veterinary Services
,
L’Oceanográfic, Ciudad de las Artes y las Ciencias, Junta de Murs i Vals, s/n, 46013
Chapters
00:05
Title
01:26
Dolphin Sperm Collection
02:44
Dolphin Sperm Evaluation and Cryopreservation
04:20
Heterologous
In Vitro
Fertilization Using Zona-intact Bovine Oocytes: Set-up
07:23
Heterologous
In Vitro
Fertilization Using Zona-intact Bovine Oocytes
09:01
Results: Analysis of Frozen Dolphin Spermatozoa and Dolphin-bovine Hybrid Embryos
10:31
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们目前成功地用于海豚精子收集、 冷冻保存和异源使用牛卵母细胞的体外受精效果的协议。
Tags
Bottlenose Dolphin
Spermatozoa
Cryopreservation
Heterologous In Vitro Fertilization
Sperm Collection
Sperm Evaluation
Sperm Motility
Sperm Viability
Sperm Morphology
Liquid Nitrogen Vapor
Assisted Reproductive Technologies
Male Pronuclear Formation
Hybrid Embryo Cleavage
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