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Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation
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Developmental Biology
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JoVE Journal Developmental Biology
Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation

English

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7,736 Views

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08:24 min

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January 28, 2018

DOI:

10.3791/56835-v

DOI:
10.3791/56835-v

•

08:24 min
January 28, 2018

•

1 Views
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1Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal,Universitat Politècnica de València, 2Department of Aquaculture, Institute of Aquaculture and Environmental Safety,Szent Istvan University

Summary

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Protocols for European eel maturation and sperm cryopreservation have been improved over the last years. This article describes the best protocol available using human chorionic gonadotropin (hCG) for inducing maturation and methanol as cryoprotectant.

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Herranz-Jusdado, J. G., Kása, E., Kollár, T., Gallego, V., Peñaranda, D. S., Rozenfeld, C., Pérez, L., Horváth, Á., Asturiano, J. F. Handling and Treatment of Male European Eels (Anguilla anguilla) for Hormonal Maturation and Sperm Cryopreservation. J. Vis. Exp. (131), e56835, doi:10.3791/56835 (2018).

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