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In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition
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JoVE Journal Developmental Biology
In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

In Vitro and In Vivo Models to Study Corneal Endothelial-mesenchymal Transition

DOI:
10.3791/54329-v

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09:05 min

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August 20, 2016

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, , , , , ,
1Department of Ophthalmology,Far Eastern Memorial Hospital, 2Institute of Clinical Medicine,National Taiwan University, 3Department of Ophthalmology,National Taiwan University Hospital, 4College of Medicine,National Taiwan University

Chapters

  • 00:05Title
  • 01:06Primary Preparation and Immunostaining of Bovine Corneal Endothelial Cells
  • 04:24Rat Corneal Endothelium Cryoinjury Model and Intracameral Injection
  • 06:46Results: Immunostaining of In Vitro Cultured Bovine CECs with Marimastat Rat Corneal Buttons After Cryoinjury
  • 08:27Conclusion

Summary

Automatic Translation

Automatic Translation

A primary culture of bovine corneal endothelial cells was used to investigate the mechanism of corneal endothelial-mesenchymal transition. Furthermore, a rat corneal endothelium cryoinjury model was used to demonstrate corneal endothelial-mesenchymal transition in vivo.

Tags

Keywords: Corneal Endothelial-mesenchymal TransitionIn VitroIn VivoCorneal EndotheliumRegenerationBovine EyesDescemet’s MembraneCorneal Endothelial CellsSHEMTrypsinHemacytometerMaramastatParaformaldehydeTriton X-100BSAABCSnailSlug

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