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新生小鼠胰腺胰岛功能的有效隔离
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Developmental Biology
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JoVE Journal Developmental Biology
Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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新生小鼠胰腺胰岛功能的有效隔离

DOI:
10.3791/55160-v

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07:04 min

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January 06, 2017

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1Department of Cell and Development Biology,Vanderbilt University Medical School

Chapters

  • 00:05Title
  • 00:39Pancreas Isolation and Digestion
  • 02:14Lysate Washing and Islet Isolation
  • 04:42Results: Representative Islet Isolation Analyses
  • 05:39Conclusion

Summary

Automatic Translation

Automatic Translation

我们在此描述了一个协议，从新生小鼠分离胰岛完好无损。胰腺被部分用胶原酶消化，接着洗涤和手采摘。 20 - 80胰岛集群可以每胰腺从新生小鼠，这是适合于几个胰岛研究而获得。

Tags

Neonatal Mouse PancreasIslet IsolationCollagenase DigestionHBSSRPMI 1640PolysucroseSodium DiatrazoateInsulin SecretionGene ExpressionParacell SurvivalFunctional Maintenance

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