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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
Capítulos
- 00:05Título
- 00:39Pancreas Isolation and Digestion
- 02:14Lysate Washing and Islet Isolation
- 04:42Results: Representative Islet Isolation Analyses
- 05:39Conclusion
We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
