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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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Effective Isolation of Functional Islets from Neonatal Mouse Pancreas
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January 06, 2017
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We describe a protocol herein for isolating intact islets from neonatal mice. Pancreata were partially digested with collagenase, followed by washing and hand picking. 20 - 80 islet clusters can be obtained per pancreas from newly born mice, which are suitable for several islet studies.
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Huang, C., Gu, G. Effective Isolation of Functional Islets from Neonatal Mouse Pancreas. J. Vis. Exp. (119), e55160, doi:10.3791/55160 (2017).
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