Production et administration de la thérapeutique mésenchymateuses souches / cellules stromales (MSC) Spheroids apprêté dans les cultures 3-D dans des conditions d'Xeno-libres
Production et administration de la thérapeutique mésenchymateuses souches / cellules stromales (MSC) Spheroids apprêté dans les cultures 3-D dans des conditions d'Xeno-libres
The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.
Ylostalo, J. H., Bazhanov, N., Mohammadipoor, A., Bartosh, T. J. Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions. J. Vis. Exp. (121), e55126, doi:10.3791/55126 (2017).