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JoVE Journal Developmental Biology
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
 

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Article DOI: 10.3791/55126-v • 11:05 min • March 18th, 2017
March 18th, 2017 •
1, 2, 3, 4
1Department of Biology, University of Mary Hardin-Baylor, 2Department of Pediatrics, University of Texas Medical Branch, 3Multi-Organ Support Technology Task Area, U.S. Army Institute of Surgical Research, 4Internal Medicine, Texas A&M University Health Science Center

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The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.

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Keywords: Mesenchymal Stem/stromal Cells MSC Spheroids Xeno-free Conditions Cell Retention Cell Survival Tissue Repair Therapeutic Efficacy 3D Culture Priming Cell Harvesting Cell Counting Spheroid Formation Spheroid Harvesting
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