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Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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JoVE Journal 発生生物学
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

DOI:
10.3791/55126-v

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11:05 min

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March 18, 2017

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, , ,
1生物学科,University of Mary Hardin-Baylor, 2小児科,University of Texas Medical Branch, 3Multi-Organ Support Technology Task Area,U.S. Army Institute of Surgical Research, 4Internal Medicine,Texas A&M University Health Science Center

章

  • 00:05標題
  • 01:25MSC Expansion and Preparation of Activated MSC Spheroids in 3-D Hanging Drop Cultures
  • 04:50Intraperitoneal Delivery of Intact Spheroids
  • 08:02Results: Spheroids Produced Using MSC Hanging Drop Cultures
  • 09:31Conclusion

概要

自動翻訳

自動翻訳

The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.

タグ

Mesenchymal Stem/stromal CellsMSC SpheroidsXeno-free ConditionsCell RetentionCell SurvivalTherapeutic Efficacy3D CulturePrimingCell HarvestingCell CountingSpheroid FormationSpheroid Harvesting

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