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Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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Gelişim Biyolojisi
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JoVE Journal Gelişim Biyolojisi
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

11,648 Views

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11:05 min

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March 18, 2017

DOI:

10.3791/55126-v

DOI:
10.3791/55126-v

•

11:05 min
March 18, 2017

•

3 Views
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1Biyoloji Bölümü,University of Mary Hardin-Baylor, 2Pediatri Anabilim Dalı,University of Texas Medical Branch, 3Multi-Organ Support Technology Task Area,U.S. Army Institute of Surgical Research, 4Internal Medicine,Texas A&M University Health Science Center

Özet

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The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.

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Cite this Article

Ylostalo, J. H., Bazhanov, N., Mohammadipoor, A., Bartosh, T. J. Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions. J. Vis. Exp. (121), e55126, doi:10.3791/55126 (2017).

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