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Uma proteína Método de Preparação para a Identificação de alto rendimento de proteínas que interagem com um co-fator nuclear utilizando LC-MS / MS Análise
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05:43 min
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January 24, 2017
DOI:
1Graduate School of Frontier Biosciences,Osaka University, 2Department of Biotechnology and Genetic Engineering,Jahangirnagar University, 3Division of Cell Regeneration and Transplantation,School of Medicine, Nihon University, 4Institute of Genetics and Animal Breeding of the Polish Academy of Sciences, 5Graduate School of Life and Medical Sciences,Doshisha University
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Nós estabelecemos um método para a purificação de proteínas de interacção co-regulação que utilizam o sistema de LC-MS / MS.
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Tsuchiya, M., Karim, M. R., Matsumoto, T., Ogawa, H., Taniguchi, H. A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis. J. Vis. Exp. (119), e55077, doi:10.3791/55077 (2017).
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