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Dekstran forbedrer Lentiviral signaltransduksjon effektiviteten av Murine og menneskelig primære NK celler
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1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute,The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute,The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute,The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute,The Blood Center of Wisconsin, 5Department of Microbiology and Immunology,The Medical College of Wisconsin, 6Department of Pediatrics,The Medical College of Wisconsin, 7Department of Medicine,The Medical College of Wisconsin
Chapters
- 00:05Title
- 00:44Purification and Expansion of Murine Primary NK Cells
- 02:03Purification and Expansion of Human Primary NK Cells
- 03:23Transduction of Murine and Human Primary NK Cells with Lentivirus
- 06:25Results: Lentiviral Transduction Efficiency Enhanced with Dextran
- 07:58Conclusion
Målet med denne studien var å formulere teknologi som tillater for vellykket genet signaltransduksjon i primære naturlig killer () Celler NK. Den dekstran-mediert lentiviral signaltransduksjon menneske eller musen primære NK celler resulterer i høyere gene expression effektivitet. Denne metoden av genet signaltransduksjon vil vesentlig forbedre NK cellen genetisk manipulasjon.
