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Dextran erhöht die Effizienz der Lentivirale Transduktion von murinen und menschliche primäre NK-Zellen
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08:42 min
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January 15, 2018
DOI:
1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute,The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute,The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute,The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute,The Blood Center of Wisconsin, 5Department of Microbiology and Immunology,The Medical College of Wisconsin, 6Department of Pediatrics,The Medical College of Wisconsin, 7Department of Medicine,The Medical College of Wisconsin
Summary
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Das Ziel dieser Studie war es, Technologien zu formulieren, die für erfolgreiche gen Transduktion in primären natürlichen killer (NK) Zellen ermöglichen. Die Dextran-vermittelten Lentivirale Transduktion von Mensch oder Maus primären NK Zellen führt zu höheren Wirkungsgraden der gen-Expression. Diese Methode des Gens Transduktion wird NK Zelle genetischen Manipulation erheblich verbessern.
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Nanbakhsh, A., Best, B., Riese, M., Rao, S., Wang, L., Medin, J., Thakar, M. S., Malarkannan, S. Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells. J. Vis. Exp. (131), e55063, doi:10.3791/55063 (2018).
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