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Dextrano mejora la eficiencia de transducción lentivirales de las células NK primaria murinas y humanas
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1Laboratory of Molecular Immunology and Immunotherapy, Blood Research Institute,The Blood Center of Wisconsin, 2Vector Core Lab, Blood Research Institute,The Blood Center of Wisconsin, 3Laboratory of Lymphocyte Biology, Blood Research Institute,The Blood Center of Wisconsin, 4Laboratory of Stem Cell Transcriptional Regulation, Blood Research Institute,The Blood Center of Wisconsin, 5Department of Microbiology and Immunology,The Medical College of Wisconsin, 6Department of Pediatrics,The Medical College of Wisconsin, 7Department of Medicine,The Medical College of Wisconsin
Chapters
- 00:05Title
- 00:44Purification and Expansion of Murine Primary NK Cells
- 02:03Purification and Expansion of Human Primary NK Cells
- 03:23Transduction of Murine and Human Primary NK Cells with Lentivirus
- 06:25Results: Lentiviral Transduction Efficiency Enhanced with Dextran
- 07:58Conclusion
El objetivo de este estudio fue elaborar tecnologías que permiten la transducción génica exitosa en natural killer (NK) las células primarias. La transducción lentivirales dextrano-mediada de humanos o resultados primarios de las células NK del ratón en una mayor eficiencia de expresión génica. Este método de transducción génica mejorará enormemente la manipulación genética de la célula NK.
