A subscription to JoVE is required to view this content. Sign in or start your free trial.
10,075 Views
•
09:15 min
•
August 19, 2016
DOI:
1Division of Nephrology,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital, 2Division of Biomedical Sciences,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital, 3Division of Endocrinology,Department of Medicine, University of Missouri-Columbia and Harry S Truman Memorial Veteran’s Hospital
Summary
Automatically generated
Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.
Read Article
Cite this Article
Nistala, R., Meuth, A., Smith, C., Annayya, A. Reliable and High Efficiency Extraction of Kidney Immune Cells. J. Vis. Exp. (114), e54368, doi:10.3791/54368 (2016).
Copy