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葡聚糖增强小鼠和人原发性 NK 细胞的慢转导效率
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
DOI:

08:42 min

January 15, 2018

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Chapters

  • 00:05Title
  • 00:44Purification and Expansion of Murine Primary NK Cells
  • 02:03Purification and Expansion of Human Primary NK Cells
  • 03:23Transduction of Murine and Human Primary NK Cells with Lentivirus
  • 06:25Results: Lentiviral Transduction Efficiency Enhanced with Dextran
  • 07:58Conclusion

Summary

Automatic Translation

这项研究的目的是制定技术, 允许成功的基因转导在原自然杀手 (NK) 细胞。葡聚糖介导的人或小鼠原代 NK 细胞的慢转导可导致较高的基因表达效率。这种基因转导方法将极大地改善 NK 细胞的遗传操作。

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