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ヒト子宮頸管ガンマ・デルタT細胞の単離およびフローサイトメトリー分析
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
DOI:

08:10 min

February 06, 2017

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Chapters

  • 00:05Title
  • 01:01Endocervical Cytobrush Sample Processing
  • 02:11Endocervical Cell Flow Cytometric Analysis
  • 03:34Gamma Delta T Cell Isolation
  • 05:37Results: Representative Human Endocervical Gamma Delta T Cell Analyses
  • 06:50Conclusion

Summary

Automatic Translation

We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.

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